3-2 Important structures in the cytoplasm

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Learning Objectives

After reading this section, students will be able to...

  1. Explain the organizational units of the cell, cytoplasm, ribosomes and nuclear region.
  2. Describe how enzymes in a pathway can be organized into structures that facilitate their activity.
  3. Identify the structure of the cytoplasm and explain that most of the metabolic reactions occur in the cytoplasm.
  4. Explain the roles of RNA in translation.
  5. Explain the roles of microscopically visible structures in the cell (inclusions, vesicles, and magnetosomes).

For a refresher, or introduction to basic chemistry and biochemistry, please read chapter 2. In this chapter, we will look at how this chemistry combines to form major functional units. These can be thought of as the components that carry out the major business of the cell: growth, replication, feeding and movement. We will describe the cell from the inside out, starting with the cytoplasm, moving to the membrane, the cell wall, and finally surface structures on microbes. Before we begin, we should describe an important evolutionary hypothesis that will make sense of much of the following details. As you will see, there are a number of curious similarities and differences in the details of cellular structure among bacteria, archaea and eukaryotes. In general much of the machinery in a eukaryotic nucleus and in the cytoplasm looks rather a lot like what is present in the archaea. However, the organelles of eukaryotes, such as the mitochondria and chloroplasts, have properties that are much more similar to those of bacteria. How is this possible? One clue comes from observing organisms in nature. It is very common to find cooperative relationships between different species and this is also true in the microbial world. In some instances these relationships involved close physical contact between their participants, sometimes with one participant engulfing the other. In 1968 Dr. Lynn Margulis extended this observation and proposed that some of the organelles found in eukaryotes, specifically mitochondria and chloroplasts, were originally endosymbionts of their host. Originally these two microbes probably were able to live independently, but over time, the endosymbiont lost functionality that its host was already providing and then became dependent. Over the years ample evidence has accumulated to support this exceptional insight.

  1. Both mitochondria and chloroplasts contain DNA that resembles the chromosomes of bacteria.
  2. Both organelles are surrounded by two membranes reminiscent of gram-negative cell wall structure (see below).
  3. Mitochondria and chloroplasts divide by a method that resembles binary fission.
  4. Much of the internal structure and biochemistry of the photosynthetic organelle inside chloroplasts is very similar to that observed in cyanobacteria (a photosynthetic microbe).
  5. The ribosomes of mitochondria and chloroplasts resemble those found in microbes and analysis of the sequence of the 16S rRNA of these ribosomes showed that the organelles are in fact closely related to proteobacteria (mitochondria) and cyanobacteria (chloroplasts) This last bit of proof is very strong evidence substantiating Dr. Margulis's hypothesis.

In summary, it seems clear that the eukaryotic cell was born by the merger of an archaeal cell with a gram-negative proteobacteria. Photosynthetic eukaryotes arose from a second endosymbiosis, where the eukaryotic cell engulfed a cyanobacterium. Clearly, eukaryotes, including us, were the result of the cooperation of several bacterial species in the long distant past. In other words, not only do you have bacteria growing on you, you are bacteria.

The cytoplasm is the area inside the membrane

The cytoplasm or protoplasm is the portion of the cell that lies within the cytoplasmic membrane. The cytoplasmic matrix is defined as substances within this membrane, excluding the genetic material. In most prokaryotes, it appears to be relatively featureless by electron microscope, but that simply means that there are no large structures we can see. This is in contrast to eukaryotic cells, which have mitochondria, and typically other visible organelles that exist for different specific functions. Despite this visual simplicity, the prokaryotic cytoplasm is the site of almost all of the important metabolic functions in the cell.

The cytoplasm has a gel-like consistency, with rather different properties than the simple solutions that we typically make up in the laboratory. This is because there is surprisingly little free water in the cell. Rather than picturing the cytoplasm as a pool of water with the occasional large molecule floating around, it is better to think of it as a bag of proteins and other macromolecules, each coated with a layer of water, and with a modest number of free water molecules bouncing around in between. In fact, there is so little free water in the cell that one-third of all water molecules are making hydrophilic contacts with the macromolecules in the cell. Given this difference between our lab solutions and the actual nature of the cytoplasm, it is a bit surprising that the biochemical analyses we perform in the lab mimic the behavior observed in the cell.

Enzymes serve as catalysts in the cytoplasm

The cytoplasm is the site of the majority of metabolism and the details of these processes are covered in the chapters on metabolism and photosynthesis. However, there are a few general issues concerning enzymes in the cytoplasm that should be mentioned here.

Though the cytoplasm appears featureless, there is actually a significant amount of local organization. A good illustration of this can be found by examining the enzymes of DNA replication. Though too small to be seen, the proteins that perform replication are in complex assemblies of many proteins. This is much more efficient that having each protein float around, and simply finding the right time and place to perform its function by random chance. For example, DNA gyrase, which unwinds and opens the DNA for copying, has to function in coordination with DNA polymerase, which inserts each new nucleotide in the growing strand. Without this coordination, the DNA would not open up for replication and the process would simply not occur. In some other processes it would certainly be possible for the enzymes to float around in the cytoplasm without any interaction, but often it is much more efficient to organize in some fashion. The glycolytic enzymes (enzymes that oxidize sugars for energy) are an example of this type of multi-enzyme complex. One enzyme directly hands over its product to the next enzyme for which it is the substrate - a sort of molecular assembly line. This is much more efficient, because substrate does not accumulate where it should not and the local concentration of substrate for each enzyme is very high.

The cell DNA is organized into a nucleoid

The nucleoid is the region of the cell that is currently occupied by the DNA-protein complex that makes up the chromosome. This is not a set region, as in eukaryotes, with their nuclei, but can vary from cell to cell. So what do we know about prokaryotic chromosomes? What does a typical chromosome look like?

It is a little awkward to talk about "typical" anything in prokaryotes, since there is always such a large range for any given property. In the case of prokaryotic chromosomes, the smallest known is about 160 kB, while the largest is about 10,000 kB. E. coliis certainly well-studied and might well be considered typical, with strain K12 having a chromosome of 4,639,221 bp containing 4405 genes. The chromosome for this bacterium is circular and this is a common arrangement, but there are a number of species with linear chromosomes. If stretched out, this material would be about 1400 µm long or about 1/16thof an inch. The E. coli cell is about 1-5 µm in length so it is clear that an amazing degree of packing is necessary to fit the chromosome inside its tiny host. Figure 3.2 shows the nucleoid of E. coli. The nucleoid occupies about half of the bacterial cytoplasm and has a density of 20-50 mg/ml, similar to what is observed for the nucleus of a non-dividing eukaryotic cell. The following description of the nucleoid refers specifically to that of E. coli, but is probably generally similar to that of other prokaryotes.

Electron micrograph of the nucleoid

Figure 3.2. Electron micrograph of the nucleoid. The nucleoid as seen in thin sections of growing E. coli. Panels A and B show the same section; in panel B the ribosome-free spaces were enhanced by coloring by hand. Panel C shows a similar cell stained with antibodies specific for DNA. (Source: E. Kellenberger).

The nucleoid is composed of DNA in association with a number of DNA-binding proteins that help it maintain its structure. The proteins HU, H-NS, and IHF are found in the DNA and help create the nucleoid's structure. HU and H-NS non-specifically binds to DNA with H-NS serving as the major DNA binding protein. IHF and HU both facilitate bending of DNA, but IHF does so by binding to specific DNA sites. The nucleoid also contains a large amount of RNA polymerase and RNA, as well as small amounts of many different proteins that regulate the expression of specific genes. These seem not to perform any structural role, but reflect the importance of RNA transcription in the nucleoid in growing cells.

The DNA double helix typically has a bit of twisting tension in the opposite direction of the helix ladder (the twists typically "unwind" the left-handed DNA helix). Negative supercoiling, as it is called, is produced by the action of enzymes termed topoisomerases. Figure 3.3 shows supercoiling of the DNA. This negative supercoiling makes it slightly easier to separate the two strands of the double helix, as must be done to start transcription and replication.

Supercoiling of DNA strands

Figure 3.3. Supercoiling of DNA strands. Supercoiling is very difficult to show in a static picture and it is hard to create a cartoon that adequately describes it. Instead consider this: Take a few feet of tubing or a garden hose and hold one end in your left hand. Then with your right hand, rotate the other end along its long axis. Since the tubing/hose is resistant to this twisting, this energy will cause a section of the tubing to form a new structure, a bit like the middle picture above. This "supertwisting" is supercoiling. Now the analogy between tubing and a DNA double-helix is not bad - both objects are able to bend but cannot really rotate much along their long axis, so if one could do the same thing with a piece of DNA, the same supertwisting would result. Of course no one is "holding one end of the DNA" in the cell, but the fact that the DNA is a closed circle has the same effect. In this picture, double-stranded DNA is shown, but for clarity the helical nature of the DNA is not depicted (though it certainly IS helical). Twist refers to the number of helical turns in the DNA and writhe to the number of times the double helix crosses over on itself (these are the supercoils). The bottom two figures shows how the supercoils could be concentrated in a single region or dispersed.

Figure 3.3 Supercoiling of DNA strands. Supercoiling is very difficult to show in a static picture and it is hard to create a cartoon that adequately describes it. Instead consider this: Take a few feet of tubing or a garden hose and hold one end in your left hand. Then with your right hand, rotate the other end along its long axis. Since the tubing/hose is resistant to this twisting, this energy will cause a section of the tubing to form a new structure, a bit like the middle picture above. This "supertwisting" is supercoiling. Now the analogy between tubing and a DNA double-helix is not bad - both objects are able to bend but cannot really rotate much along their long axis, so if one could do the same thing with a piece of DNA, the same supertwisting would result. Of course no one is "holding one end of the DNA" in the cell, but the fact that the DNA is a closed circle has the same effect. In this picture, double-stranded DNA is shown, but for clarity the helical nature of the DNA is not depicted (though it certainly IS helical). Twist refers to the number of helical turns in the DNA and writhe to the number of times the double helix crosses over on itself (these are the supercoils). The bottom two figures shows how the supercoils could be concentrated in a single region or dispersed.

As shown in Figure 3.4,the chromosome is further folded into 50 or so loops of about 100,000 base pairs. These domains supercoil independently and indeed, even small sections within the same loop can transiently have different degrees of supercoiling. It does appear that supercoiling can affect the expression of genes in a region of the DNA and that gene expression also affects supercoiling. Because supercoiling changes fairly rapidly, it has been extremely difficult to study in living cells. In addition to this rosette morphology, one or several loci of the chromosome are specifically positioned within the cell and the intervening DNA is kept organized with respect to these landmarks. In fact, you will find specific regions of the chromosome in similar positions in the cell. How this is specifically arranged is species-specific, with different microbes arranging their chromosomes in different ways. For example, E. colikeeps its oriCat the center of the cell, and has left and right halves of the chromosome that flow outward from there, eventually meeting at the terminus. Caulobacterspecies, curved rod-shaped microbes, will keep their origin and terminus at opposite poles of the cell.

Chromosome structural organization

Figure 3.4. Chromosome structural organization. A model of the overall structure of the bacterial chromosome. (A) The unfolded, circular chromosome of E. colidepicted as a single line for simplicity, though of course it is a double-stranded helix. (B) The DNA folded into chromosomal domains by protein-DNA associations. The proteins are depicted as the black circles, interacting with both the DNA and with each other. Six domains are shown, but the actual number for E. coli is about 50. (C) Supercoiling and other interactions cause the chromosome to compact greatly.

Transcription and translation occur on the surface of the nucleoid

So we have told you that the DNA in the cell has a very compact structure because of DNA-binding proteins, but we have also said that that structure is very dynamic because gene transcription is going on all of the time. This paradox creates a very difficult situation for the cell that only becomes worse when it is time to replicate the chromosome. Remember that prokaryotes continue to perform gene expression throughout replication, in contrast to eukaryotes. As you will learn below, transcription and translation are coupled in bacteria - the beginning of the messenger RNA (mRNA), termed the 5' end, is being actively translated while last portion, termed the 3' end, is still being synthesized. This process is depicted in Figure 3.5. Yet the internal regions of the nucleoid appear to be devoid of ribosomes and non DNA-binding proteins, suggesting that all transcription and translation must occur on the surface of the nucleoid. Therefore the cell must shift this large tangle of DNA around by some unknown mechanism as gene expression of certain buried sequences is needed. It is a marvel that the whole process of transcription, translation and replication works at all, especially within the very small confines of the cell.

The coupling of transcription and translation

Figure 3.5. The coupling of transcription and translation. In prokaryotes the process of transcription and translation are tightly coupled. This increases the rate at which proteins can be expressed and is one reason that some bacteria can multiply so quickly.

Translation involves messenger RNA

Translation is the process of converting the instructions coded in the DNA into the proteins that actually carry out the work. The macromolecules that perform this task consist of mRNA, transfer RNA (tRNA) and the ribosome, which is made of ribosomal RNA (rRNA) and ribosomal proteins. In brief, this process consists of making an mRNA copy of a region in the DNA that gives directions for the synthesis of a protein or proteins. The mRNA is then bound by a ribosome that translates the mRNA into a amino acid sequence. The amino acids necessary for the protein are carried to the ribosome by tRNA that actually read the information in the mRNA and add the appropriate amino acid to the nascent protein chain. This is described in much greater detail in the chapter on the central dogma.

We will now examine the structure of the molecules involved in translation, starting with mRNA, where the primary structure is simple - merely unmodified A, G, C and U bases. In almost all prokaryotic mRNAs there is not a great deal of secondary and tertiary structure, since they are typically being translated by ribosomes and the translating ribosome certainly removes any structure as it moves along the mRNA. What structure there is tends to be in the regions that are not translated, notably the 5' and 3' ends of the mRNA. One of the roles of structure, especially at the 3' end, is to stabilize the mRNA. There are RNases in the cell that degrade the RNA and RNA secondary structure can impede this degradation. The amount of secondary structure is by design, depending upon how long the cell wants the mRNA to last. Due to the need of most prokaryotic cells to respond rapidly to changes in the environment, most mRNAs are not very stable in the cell and are rapidly degraded.

Ribosomes are composed of RNA and protein

In contrast to the case with mRNAs, the other RNAs involved in translation, tRNA and rRNA, have very distinct structures. Each rRNA folds into a known secondary structure and has a complex tertiary structure containing many short helical regions and long range base pair interactions. These structures are also maintained by interactions between the RNAs and protein.

The composition of ribosomes is 62 % RNA and 38 % protein by weight. Two complexes of RNA and protein make up the ribosome, the 30S subunit and the 50S subunit. (The S stands for Svedberg units, a measure of how fast something sediments in solution. For our purposes all you need to know is that larger molecules sediment faster and have larger Svedberg units. The unit is named after Theodor Svedberg who won the Nobel Prize in Chemistry in 1926 for his work on suspensions of large molecules and other compounds in solutions.) The 30S subunit is composed of 21 proteins and a single-stranded rRNA molecule of about 1,500 nucleotides, termed the 16S rRNA. The 50S subunit contains 31 proteins and two RNA species, a 5S rRNA of 150 nucleotides and a 23S rRNA of about 2,900 nucleotides. Several of the nucleotides on the 16S and 23S rRNAs have been modified by methylation and these modifications are probably critical to the function of the rRNAs since they always happen in regions conserved through evolution.

The ribosome-associated proteins are positively charged, with a high proportion of lysine and arginine residues. This facilitates complex formation between the acidic RNA and these basic proteins. The crystal structure of the entire 70S ribosome has been solved as shown in Figure 3.6, which is quite remarkable because solving such structures becomes more difficult as the size of the complex increases and the ribosome is huge. We are still trying to fully understand what that structure tells us about ribosome function. We also know that the ribosome is not static, but dynamic, changing shape during a translation cycle.

The crystal structure of the 70S ribosome.

Figure 3.6. The crystal structure of the 70S ribosome.. A molecular model of the 70S ribosome of Thermus thermophilis showing the position of proteins (purple and red ribbon diagrams) and RNA (Gray and cyan ribbons) within the structure. Note the prominence of RNA in the ribosome, which constitutes over 60% of the molecular weight. (Source: adaption from S. Petry et al. 2005. Cell 123:1255-1266)

Transfer RNA is the ferry for amino acids

Transfer RNA (tRNA) is the ferry that transports the amino acids to the ribosome. There are one or more different tRNA molecules for each of the 20 amino acids. Each consists of 70 to 80 nucleotides of single-stranded RNA that is extensively base-paired to form four short helical domains. These structures are commonly represented as a two-dimensional cloverleaf, but look more like an "L" in the native three-dimensional structure as shown in Figure 3.7. Now the tertiary structures of tRNAs are all rather similar, so the critical features that make each appropriate to a specific amino acid are largely found in the primary structure itself. Many bases in tRNA molecules are chemically modified by enzymes to help the molecule carry out its function.

The structure of tRNA

Figure 3.7. The structure of tRNA. The two-dimensional structure of tRNA looks like a cloverleaf, but in the actual three-dimensional form, it has a surprising L-shaped structure as shown. The bottom of the structure as shown here contains the anticodon that interacts with the mRNA. The top right of the structure is where the appropriate amino acid residue is attached by synthetases.

The aminoacyl-tRNA synthetases are the enzymes that add the amino acid to the tRNAs. Figure 3.8 depicts a complex between an aminoacyl tRNA synthetase and tRNA. There is a single synthetase for each amino acid and it binds each of its appropriate tRNA molecules and charges it with its appropriate amino acid. The synthetases avoid both the wrong tRNAs as well as the wrong amino acids.

The amino acyl-tRNA synthetase complex

Figure 3.8. The amino acyl-tRNA synthetase complex. A molecular model of aminoacyl tRNA synthetase binding its tRNA. The tRNA is shown as ball-and-stick, while the synthetase is depicted in the ribbon form. In this picture, the anticodon is at the top of the figure and the site of amino acid attachment is at the bottom right. Note that the synthetase does not "sense" the anticodon directly.

One final thought before we move on. Think about the chicken-and-egg conundrum that translation brings up. This whole process has the express purpose of synthesizing proteins, yet it involves proteins at every step. How could these proteins have evolved to serve this function when they are needed for their own synthesis? In other words, how could you synthesize anyprotein until a complete set of translation proteins had evolved? Part of the answer is that primordial translation was probably much simpler, though less accurate and efficient. Perhaps only very few and somewhat general protein functions were actually required. Alternatively, perhaps early translation used no proteins at all: Some scientists believe that early life employed RNA molecules that were capable of both carrying out necessary enzymatic functions and storing hereditary information. They posit that it was only later that proteins came along and started to assist in their own synthesis. However, this hypothesis still does not explain how one simultaneously evolved functional proteins and a process for creating them.

Inclusions and other internal structures are found in many prokaryotic cells

There are a few structures in the prokaryotic cytoplasm that are visible by microscopy.In general, they serve specific purposes in the cell and are often found only in certain cell types or under certain growth conditions. Here we focus on some of the more familiar objects, inclusions, gas vesicles and magnetosomes.


Inclusions are dense aggregates of specific chemical compounds in the cell. Typically, the aggregated chemical serves as a reservoir of either energy-rich compounds or building blocks for the cell. Forming polymers costs energy and it may seem wiser for the cell to keep the excess monomers around for when they are needed. The benefit of polymerization is that it decreases the osmotic pressure on the cell, a serious problem as described later. Inclusions often accumulate under laboratory conditions when a cell is grown in the presence of excess nutrients. However, the role of some inclusions is unclear. Growth on rich medium causes their creation, but subsequent starvation in the test tube does not always result in the use of these reserves. This suggests that these inclusions, at least, are not storage bodies or that we have not found conditions where the cell will use them.

Carbon and energy storage

One of the more common storage inclusions involves poly-β-hydroxyalkanoate(PHA). It is a long polymer of repeating hydrophobic units that can have various carbon chains attached to it. The most common form of this class of polymers is poly-β-hydroxybutyrate, which has a methyl group as the side chain to the molecule as shown in Figure 3.9. The function of PHA in bacteria is as a carbon and energy storage product. Just as we store fat, some bacteria store PHA. Some PHA polymers have plastic-like qualities and there is some interest in exploiting them as a form of biodegradable plastic.

Poly-β-hydroxyalkanoate inclusion bodies

Figure 3.9. Poly-β-hydroxyalkanoate inclusion bodies. The figure shows an electron micrograph of inclusion bodies of poly-β-hydroxyalkanoate inside a cell of a Rhodobacter sphaeroides. The specific chemical here is poly-β-hydroxybutyrate (PHB). In the generalized poly-β-hydroxyalkanoate structure shown at the left, the R group is a methyl in PHB. (Source, Sam Kaplan, University of Texas - Houston Medical School)

Glycogen is another common carbon and energy storage product. Humans also synthesize and utilize glycogen, which is a polymer of repeating glucose units.

Phosphate granules and sulfur globules

Given the opportunity, many organisms accumulate granules containing long chains of phosphate, since this is often a limiting nutrient in the environment. These polyphosphate polymers, also called volutin, form visible granules in some microbes. These granules are readily stained by many basic dyes such as toluidine blue and turn reddish violet in color. These inclusions are often referred to as metachromatic granules because they become visible by "metachromasy" (a color change). Polyphosphate is found in all known cells (eukaryotes, bacteria and archaea) and appears to serve many important roles.

  1. It serves as a phosphate reservoir
  2. It is an alternative substrate in place of ATP when phosphorylating sugars during catabolism.
  3. It is a chelator for divalent cations
  4. It can be a buffer under alkaline stress
  5. It is an important factor for DNA uptake.
  6. Finally, phosphate polymers are important regulators in response to stress

Figure 3.10 depicts another visible structure, termed a sulfur globule, which is found in a variety of bacteria capable of oxidizing reduced sulfur compounds such as hydrogen sulfide and thiosulfate. Oxidation of these compounds is linked either to energy metabolism or photosynthesis. Oxidation of sulfide initially yields elemental sulfur, which accumulates in globules inside or outside the cell. If the sulfide is exhausted the sulfur may be further oxidized to sulfate.

Sulfur globules

Figure 3.10. Sulfur globules. Sulfur globules found in Thiomargarita namibiensis. This large microbe (100 to 750 µm in size) is found in Walvis Bay off the coast of Namibia. (Source: copyright Max Planck Institute for Marine Microbiology, Bremen, Germany)

Gas vesicles

Figure 3.11 shows an example of gas vesicles, also known as gas vacuoles, that are found in cyanobacteria. Cyanobacteria are photosynthetic and live in lakes and oceans. In these environments, the cyanobacteria use gas vesicles to control their position in the water column to obtain the optimum amount of light and nutrients.

Gas vesicles

Figure 3.11. Gas vesicles. The hexagonal forms inside the cytoplasm of this cyanobacterium are the gas vesicles. These actively dividing cells are Microscystissp.(Source: A. E. Walsby, 1994. Microbiol. Rev. 58:94-144)

Gas vesicles are often aggregates of hollow cylindrical structures composed of rigid proteins. They are impermeable to water, but permeable to gas. The amount of gas in the vesicle is under the control of the microorganism. Release of gas from the cell causes the bacteria to fall in the water column, while filling the vesicle with gas causes the cells to rise.


Magnetosomes are intracellular crystals of iron magnetite (Fe3O4) that impart a permanent magnetic dipole to prokaryotic cells that have them. They allow these microbes to orient themselves in a magnetic field. This process does not appear to involve any special machinery beside the magnetosome, Each microbe can be thought of as having a tiny magnet that is responding to the magnetic field in the environment. These magnetosomes allow the microbes to follow the magnetic field of the earth. Some species of magnetotatic bacteria have the following behavior. In the northern hemisphere magnetotatic bacteria swim north along the magnetic field, while in the southern hemisphere they swim south. Because of the inclination of the earth's magnetic field, this causes the microbes to swim downward. Many microbes containing magnetosomes are aquatic organisms that do not grow well in the presence of atmospheric concentrations of oxygen and they move away from the oxygen higher up in the water column by detecting the magnetic field and swimming downward.

A special membrane surrounds magnetosomes that confines the magnetite to a defined area. The membrane likely plays a role in precipitating the iron as Fe3O4 in the developing magnetosome. Magnetosomes can be square, rectangular or even spike-shaped.

Key Takeaways

  1. The cytoplasm is that area inside the membrane and has a gel-like consistency.
  2. While there are no visible structures in the cytoplasm in prokaryotes, it is still highly organized. As an example, the nucleoid of the cell, containing the chromosome, is bound by proteins and supercoiled to tightly pack the DNA into the cell.
  3. Translation occurs at the ribosome and converts the recipe of the protein, encoded in DNA, and mRNA into protein.
  4. This process also involves another RNA, tRNA, as an adapter.
  5. The cell cytoplasm does contains some visible structures, called inclusions. These structures function as nutrient storage (PHA, sulfur and phosphorus globules), buoyancy (gas vesicles), and sensing the earth's magnetic field (magnetosomes).
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1. Which of the following are true concerning translation?

A. Ribosomes load onto mRNA at the 5' end and translate in the 3' direction.
B. Translating ribosomes will necessarily open up any secondary structure in the mRNA, at least for the portions that are actually translated.
C. The 3' exonucleases do not have much effect on translation because the ribosomes begin translating at the other end.
D. In bacteria, mRNAs are primarily an exact copy of of a DNA strand except that some bases can be added to the end.

2. The bacterial chromosome does not contain any organization of structure.


3. With gas vesicles, the release of gas cause the bacteria to fall in the water column?


4. Magnetosomes sense

A. Maggots, which are predators of bacteria
B. Water pressure
C. Air pressure
D. The earth's magnetic field

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